FAS Antikörper (AA 26-96)
Kurzübersicht für FAS Antikörper (AA 26-96) (ABIN6939781)
Target
Alle FAS Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Bindungsspezifität
- AA 26-96
-
Spezifität
- This MAb specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45 kDa, containing 8 kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.
-
Aufreinigung
- Purified by Protein A/G
-
Immunogen
- Recombinant fragment (around aa26-96) of human CD95 (FAS) protein (exact sequence is proprietary)
-
Isotyp
- IgG2b kappa
-
-
-
-
Applikationshinweise
-
Positive Control: MCF-7 cells (IF/FACS). Human hepatocellular or bladder carcinoma (IHC).
Known Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Konzentration
- 200 μg/mL
-
Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Lagerung
- 4 °C,-80 °C
-
Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
-
Haltbarkeit
- 24 months
-
-
- FAS (TNF Receptor Superfamily, Member 6 (FAS))
-
Andere Bezeichnung
- FAS
-
Molekulargewicht
- 38-50kDa
-
Gen-ID
- 355
-
UniProt
- P25445
-
Pathways
- p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
-