14-3-3 sigma/SFN Antikörper

Produktnummer ABIN6939540

Dieses Anti-14-3-3 sigma/SFN-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von 14-3-3 sigma/SFN in WB, IHC und StM. Geeignet für Human.

Kurzübersicht für 14-3-3 sigma/SFN Antikörper (ABIN6939540)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser 14-3-3 sigma/SFN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: CPTC-SFN-2

Produktdetails anti-14-3-3 sigma/SFN Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human full-length SFN protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: PC-3 cells, Skin and tissues enriched in stratified squamous keratinizing epithelium. HaCaT, hTCEpi, A-431 cell lines.

Known Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Andere Bezeichnung: SFN

Hintergrund: SFN (stratifin) is a p53-induced tumor suppressor gene which is activated in response to DNA damage, causing cell cycle arrest at G2 phase by blocking cdc2-cyclin B1 complex from entering the nucleus. It is inactivated in breast, lung, prostate, liver and gastric cancer. It is associated with poor prognosis when its down-regulation is observed in epithelial ovarian cancer. SFN expression could contribute to cancer cell proliferation and the development and/or progression of human gastrointestinal cancer.

Molekulargewicht: 28kDa

Gen-ID: 2810

UniProt: P31947

Pathways: p53 Signalweg, Myometrial Relaxation and Contraction