ERCC1 Antikörper (AA 191-281)

Produktnummer ABIN6939333

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ERCC1 in IHC und StM. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ERCC1 Antikörper (AA 191-281) (ABIN6939333)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERCC1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: ERCC1-2318

Produktdetails anti-ERCC1 Antikörper

Bindungsspezifität: AA 191-281

Spezifität: Recognizes a protein of 110 kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 191-281) of human ERCC1 protein (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa, HepG2 cells. Tonsil or Prostate.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Andere Bezeichnung: ERCC1

Molekulargewicht: 110kDa

Gen-ID: 2067

UniProt: P07992

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response