Rekombinanter EPO Antikörper (AA 28-162)

Produktnummer ABIN6939314

Produktdetails anti-EPO Antikörper

Target: EPO (Erythropoietin (EPO))

Antikörpertyp: Recombinant Antibody

Bindungsspezifität: AA 28-162

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPO Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Coating (Coat), Staining Methods (StM)

Spezifität: Recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 28-162) of human EPO protein (exact sequence is proprietary)

Klon: REPO-1367

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 cells. Heart or Kidney.

Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), ,Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu EPO

Target: EPO (Erythropoietin (EPO))

Andere Bezeichnung: EPO (EPO Produkte)

Substanzklasse: Hormone

Molekulargewicht: 37kDa

Gen-ID: 2056

UniProt: P01588

Pathways: JAK-STAT Signalweg, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity