Rekombinanter Thymidine Phosphorylase Antikörper

Produktnummer ABIN6939278

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch Thymidine Phosphorylase in IHC und StM. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für Rekombinanter Thymidine Phosphorylase Antikörper (ABIN6939278)

Target: Thymidine Phosphorylase (TYMP)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Thymidine Phosphorylase Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Staining Methods (StM)

Klon: TYMP-2890R

Produktdetails anti-Thymidine Phosphorylase Antikörper

Spezifität: Recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HUVEC cells. Breast, Bladder, Lung or Kaposi tumors.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Andere Bezeichnung: TYMP

Molekulargewicht: 55kDa

Gen-ID: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2