alpha Fetoprotein Antikörper

Produktnummer ABIN6939237

Der Maus Monoklonal Anti-alpha Fetoprotein-Antikörper wurde für IHC, IF, FACS und StM validiert. Er ist geeignet, alpha Fetoprotein in Proben von Human, Schwein, Hund und Affe zu detektieren.

Kurzübersicht für alpha Fetoprotein Antikörper (ABIN6939237)

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human, Schwein, Hund, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: SPM334

Produktdetails anti-alpha Fetoprotein Antikörper

Spezifität: It recognizes an oncofetal glycoprotein with a single chain of 70 kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Keine Kreuzreaktivität: Rind (Kuh), Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Alpha feto protein (AFP) Purified from serum of a hepatoma patient

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: AFP

Molekulargewicht: 70kDa

Gen-ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process