Rekombinanter ADRP Antikörper (AA 249-376)

Produktnummer ABIN6939160

Der Kaninchen Monoklonal Anti-ADRP-Antikörper wurde für WB, IHC, ELISA, Coat und StM validiert. Er ist geeignet, ADRP in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter ADRP Antikörper (AA 249-376) (ABIN6939160)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ADRP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Klon: ADFP-2755R

Produktdetails anti-ADRP Antikörper

Bindungsspezifität: AA 249-376

Spezifität: Recognizes a protein of 48 kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Known Application: ELISA (For coating, order Ab without BSA),Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Andere Bezeichnung: PLIN2

Molekulargewicht: 48kDa

Gen-ID: 123

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism