WT1 Antikörper

Produktnummer ABIN6938780

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch WT1 in IHC. Es zeigt Reaktivität gegenüber Proben von Human und Ratte.

Kurzübersicht für WT1 Antikörper (ABIN6938780)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Produktdetails anti-WT1 Antikörper

Verwendungszweck: Wilm’s Tumor 1 (WT1) (Wilm’s Tumor & Mesothelial Marker) Antibody

Spezifität: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Aufreinigung: 200ug/ml of Ab Purified by Protein A/G.

Immunogen: Recombinant human WT1 protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K562 cells. Wilm's Tumor, mesothelioma or fetal kidney.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)

Hintergrund:

WT1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1

K562 cells. Wilm's Tumor, mesothelioma or fetal kidney.

Molekulargewicht: 47-55kDa

Gen-ID: 7490, 591980

UniProt: P19544

Pathways: Tube Formation