VTCN1 Antikörper

Produktnummer ABIN6938766

Der Maus Monoklonal Anti-VTCN1-Antikörper wurde für WB, ELISA, FACS, IF, Coat und IHC (f) validiert. Er ist geeignet, VTCN1 in Proben von Human zu detektieren.

Kurzübersicht für VTCN1 Antikörper (ABIN6938766)

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VTCN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: B7H4-1788

Produktdetails anti-VTCN1 Antikörper

Spezifität: T cell activation and immune function are regulated by the innate immune system through positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research suggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Flow Cytometry (1-2 μg/million cells in 0.1ml), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa, SKBr-3 or MCF-7 cells. Human ovary, pancreas, placenta or spleen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu VTCN1

Target: VTCN1 (V-Set Domain Containing T Cell Activation Inhibitor 1 (VTCN1))

Andere Bezeichnung: VTCN1

Hintergrund: B7 family member, H4, B7 homolog 4, B7 superfamily member 1, B7-H4, B7S1, Immune costimulatory protein B7-H4, T-cell costimulatory molecule B7x, V-set domain-containing T-cell activation inhibitor 1, VCTN1,B7-H4 (Immuno-Inhibitory Protein)
Cellular localisation: Cell Surface and Cytoplasmic

Molekulargewicht: 35kDa

Gen-ID: 79679, 546434

UniProt: Q7Z7D3