Rekombinanter Vimentin Antikörper

Produktnummer ABIN6938759

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch Vimentin in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Schwein, Rind (Kuh), Huhn, Hund, Cat und Pferd.

Kurzübersicht für Rekombinanter Vimentin Antikörper (ABIN6938759)

Target: Vimentin (VIM)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Schwein, Rind (Kuh), Huhn, Hund, Cat, Pferd

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: VIM-1937R

Produktdetails anti-Vimentin Antikörper

Verwendungszweck: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Spezifität: This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Kreuzreaktivität (Details): Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Vimentin protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: U-87, A375, Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: VIM

Hintergrund:

VIM

A375, Jurkat or HeLa cells. Sarcomas or Melanomas., Raji, U-87

Molekulargewicht: 57-60kDa

Gen-ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose