Vimentin Antikörper
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- Target Alle Vimentin (VIM) Antikörper anzeigen
- Vimentin (VIM)
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Reaktivität
- Schwein
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Vimentin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
- Kreuzreaktivität (Details)
- Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Porcine Lens
- Klon
- V9
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product VIM Primärantikörper
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- Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: U-87, Raji, Jurkat or HeLa cells. Lymph node or tonsil.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Vimentin (VIM)
- Andere Bezeichnung
- VIM (VIM Produkte)
- Synonyme
- CTRCT30 antikoerper, cb28 antikoerper, vime antikoerper, vim antikoerper, vim1 antikoerper, vim2 antikoerper, VIM antikoerper, Vimentin antikoerper, vim4 antikoerper, vimentin antikoerper, vimentin L homeolog antikoerper, vimentin S homeolog antikoerper, VIM antikoerper, Vim antikoerper, vim antikoerper, vim.L antikoerper, vim.S antikoerper
- Hintergrund
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VIM,Vimentin (Mesenchymal Cell Marker)
Cellular localisation: Cytoplasmic - Molekulargewicht
- 57-60kDa
- Gen-ID
- 7431, 455493
- UniProt
- P08670
- Pathways
- Caspase Kaskade in der Apoptose
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