Rekombinanter Villin 1 Antikörper (AA 179-311)

Produktnummer ABIN6938750

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Villin 1 in IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter Villin 1 Antikörper (AA 179-311) (ABIN6938750)

Target: Villin 1 (VIL1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Villin 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RVIL1-1325

Produktdetails anti-Villin 1 Antikörper

Bindungsspezifität: AA 179-311

Spezifität: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A recombinant fragment (around aa179-311) of human Villin protein (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Villin 1

Target: Villin 1 (VIL1)

Andere Bezeichnung: VIL1

Hintergrund: VIL1, Villin-1, Villin1,Villin (GI-Mucosal & Urogenital Brush Border Marker)
Cellular localisation: Cytoplasmic & Cell Surface

Molekulargewicht: 93kDa

Gen-ID: 7429, 654595

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization