TNC Antikörper

Produktnummer ABIN6938598

Der Maus Monoklonal anti-TNC Antikörper wird verwendet zum Nachweis von TNC in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für TNC Antikörper (ABIN6938598)

Target: TNC (Tenascin C (TNC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNC Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: T2H5

Produktdetails anti-TNC Antikörper

Verwendungszweck: Tenascin C (Stromal Marker for Epithelial Malignancy) Antibody

Spezifität: In Western blotting, it reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Keine Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with Rat.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human breast carcinoma

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (2-4 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ,Optimal dilution for a specific application should be determined.

Positive_Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu TNC

Target: TNC (Tenascin C (TNC))

Andere Bezeichnung: TNC

Hintergrund:

Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion (HXB), JI, Myotendinous antigen, Neuronectin, TNC

Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell and lung carcinomas. Staining of normal fibroblasts serves as internal positive control.

Molekulargewicht: 210kDa and 300kDa

Gen-ID: 3371, 143250

UniProt: P24821

Pathways: Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development