Transferrin Receptor Antikörper (AA 94-212)

Produktnummer ABIN6938531

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Transferrin Receptor in WB, ELISA, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Transferrin Receptor Antikörper (AA 94-212) (ABIN6938531)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transferrin Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: TFRC-1817

Produktdetails anti-Transferrin Receptor Antikörper

Bindungsspezifität: AA 94-212

Spezifität: It recognizes a ~90-95 kDa protein which is identified as cell surface transferrin receptor (CD71), a disulfide-bonded homodimeric glycoprotein of 180-190 kDa. This MAb is highly specific to CD71 and shows no cross-reaction with other related proteins. Ligand for transferrin receptor is the serum iron transport protein, transferrin. This receptor is broadly distributed in carcinomas, sarcomas, leukemias, and lymphomas. CD71/Transferrin receptor has been reported to be associated with cell proliferation in both normal and neoplastic tissues and useful in predicting clinical behavior or response to therapy in a number of malignancies including breast cancer.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant extracellular fragment (around aa 94-212) of human TFRC protein (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, HeLa, MCF-7 or K562 cells. Human placenta or Bone Marrow.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Andere Bezeichnung: TFRC

Hintergrund: Mtvr-1, p90, TFR1, TFRC transferrin receptor (p90 CD71), TRFR,CD71 / Transferrin Receptor (TFRC) (Extracellular Domain)
Cellular localisation: Cell surface

Molekulargewicht: 85-95kDa (monomer), 190kDa (dimer)

Gen-ID: 7037, 529618

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis