Spectrin beta Chain, Erythrocyte (SPTB) Antikörper

Produktnummer ABIN6938442

Der Maus Monoklonal Anti--Antikörper wurde für WB, IF, IHC, ELISA, Coat und IHC (f) validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für Spectrin beta Chain, Erythrocyte (SPTB) Antikörper (ABIN6938442)

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RG-26

Produktdetails

Spezifität: Spectrin is a cytoskeletal protein that lines the intracellular side of the plasma membrane of many cell types in pentagonal or hexagonal arrangements, forming a scaffolding and playing an important role in maintenance of plasma membrane integrity and cytoskeletal structure. In certain types of brain injury such as diffuse axonal injury, spectrin is irreversibly cleaved by the proteolytic enzyme calpain, destroying the cytosketelon. Spectrin cleavage causes the membrane to form blebs and ultimately to be degraded, usually leading to cell death.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Red cell membranes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), ,Immunofluorescence (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) ,(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat whole cell lysate (WB). Human colon carcinoma tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Antigendetails

Target: Spectrin beta Chain, Erythrocyte (SPTB)

Andere Bezeichnung: SPTB

Hintergrund: Beta-I spectrin, EL3, erythrocyte, HS2, SPTB1, HSPTB1, membrane cytoskeletal protein, spectrin beta (erythrocytic),Spectrin beta I (SPTB)
Cellular localisation: Cell Surface

Molekulargewicht: 246kDa

Gen-ID: 6710, 26915

UniProt: O15020

Pathways: Regulation of Actin Filament Polymerization