Rekombinanter SPTA1 Antikörper (AA 356-475)

Produktnummer ABIN6938438

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SPTA1 in WB und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter SPTA1 Antikörper (AA 356-475) (ABIN6938438)

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SPTA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RSPTA1-1832

Produktdetails anti-SPTA1 Antikörper

Bindungsspezifität: AA 356-475

Spezifität: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human SPTA (around aa356-475) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562. HeLa, Jurkat cells. Placenta or Tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu SPTA1

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Andere Bezeichnung: SPTA1

Hintergrund: Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1,Spectrin Alpha 1 (Erythrocyte Marker)
Cellular localisation: Cytoplasmic and Cell Surface

Molekulargewicht: 240kDa

Gen-ID: 6708, 119825

UniProt: P02549

Pathways: Regulation of Actin Filament Polymerization