Spastin Antikörper

Produktnummer ABIN6938422

Produktdetails anti-Spastin Antikörper

Target: Spastin (SPAST)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Spastin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Spastin protein.

Klon: Sp 3G11-1

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Rat brain extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Spastin

Target: Spastin (SPAST)

Andere Bezeichnung: SPAST (SPAST Produkte)

Synonyme: CG5977 antikoerper, D-spastin antikoerper, Dmel\\CG5977 antikoerper, Dspastin antikoerper, Spas antikoerper, zgc:85952 antikoerper, SPAST antikoerper, spg4 antikoerper, LOC100224472 antikoerper, ADPSP antikoerper, FSP2 antikoerper, SPG4 antikoerper, Spg4 antikoerper, mKIAA1083 antikoerper, spastin antikoerper, spastin S homeolog antikoerper, spas antikoerper, spast antikoerper, SPAST antikoerper, Spast antikoerper, spast.S antikoerper

Hintergrund: ADPSP, FSP2, Spast, Spatin, SPG4,Spastin
Cellular localisation: Cell Membrane and Secreted.

Molekulargewicht: 52kDa

Gen-ID: 6683, 468091

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size