Spastin Antikörper

Produktnummer ABIN6938422

Der Maus Monoklonal anti-Spastin Antikörper (Klon Sp 3G11-1) (ABIN6938422) detektiert spezifisch Spastin in WB, ELISA, IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus, Ratte und Human.

Kurzübersicht für Spastin Antikörper (ABIN6938422)

Target: Spastin (SPAST)

Reaktivität: Maus, Ratte, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Spastin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: Sp 3G11-1

Produktdetails anti-Spastin Antikörper

Verwendungszweck: Spastin Antibody

Spezifität: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Spastin protein.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Rat brain extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Spastin

Target: Spastin (SPAST)

Andere Bezeichnung: SPAST

Hintergrund:

ADPSP, FSP2, Spast, Spatin, SPG4

HeLa cells. Rat brain extract.

Molekulargewicht: 52kDa

Gen-ID: 6683, 468091

UniProt: Q9UBP0

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size