Rekombinanter SOX10 Antikörper (AA 115-269)
-
- Target Alle SOX10 Antikörper anzeigen
- SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))
- Antikörpertyp
- Recombinant Antibody
-
Bindungsspezifität
- AA 115-269
-
Reaktivität
- Human
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser SOX10 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.
- Kreuzreaktivität (Details)
- Human. Mouse.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- A recombinant human SOX10 protein fragment (around aa115-269) (exact sequence is proprietary)
- Klon
- RSOX10-1074
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product SOX10 Primärantikörper
-
-
- Applikationshinweise
-
Known_Application: Western Blot (2-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: COLO-38 or Melanomas, breast carcinomas, gliomas (IHC).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
-
- Target
- SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))
- Andere Bezeichnung
- SOX10 (SOX10 Produkte)
- Synonyme
- DOM antikoerper, PCWH antikoerper, WS2E antikoerper, WS4 antikoerper, WS4C antikoerper, SOX-10 antikoerper, SOX10 antikoerper, dom antikoerper, ws4 antikoerper, Dom antikoerper, Sox21 antikoerper, SOX9 antikoerper, cls/sox10 antikoerper, zgc:100757 antikoerper, SRY-box 10 antikoerper, SRY box 10 antikoerper, SRY-box 10 L homeolog antikoerper, SRY (sex determining region Y)-box 10 antikoerper, SOX10 antikoerper, Sox10 antikoerper, sox10.L antikoerper, sox10 antikoerper
- Hintergrund
-
DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C),SOX10 (Melanoma Marker)
Cellular localisation: Nuclear - Molekulargewicht
- 49-58kDa
- Gen-ID
- 6663, 376984
- UniProt
- P56693
- Pathways
- Chromatin Binding
-