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Melanoma gp100 Antikörper

PMEL Reaktivität: Human IF, FACS, IHC (f) Wirt: Maus Monoclonal SPM286 unconjugated
Produktnummer ABIN6938387
  • Target Alle Melanoma gp100 (PMEL) Antikörper anzeigen
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    Reaktivität
    • 87
    • 12
    • 7
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Wirt
    • 47
    • 37
    • 1
    • 1
    Maus
    Klonalität
    • 48
    • 38
    • 1
    Monoklonal
    Konjugat
    • 48
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Melanoma gp100 Antikörper ist unkonjugiert
    Applikation
    • 39
    • 39
    • 37
    • 36
    • 24
    • 13
    • 7
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Spezifität
    By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
    Keine Kreuzreaktivität
    Ratte (Rattus)
    Kreuzreaktivität (Details)
    Human. Horse. Does not react with Rat.
    Aufreinigung
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Membranes from a human melanoma metastasis
    Klon
    SPM286
    Isotyp
    IgG2b kappa
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    Discover our top product PMEL Primärantikörper
  • Applikationshinweise

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: SK-MEL-28 cells. Melanoma.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Haltbarkeit
    24 months
  • Target
    Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))
    Andere Bezeichnung
    SILV (PMEL Produkte)
    Hintergrund
    95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog,Melanosome gp100 / PMEL17 / SILV (Melanoma Marker)
    Cellular localisation: Cytoplasmic
    Molekulargewicht
    90-100kDa
    Gen-ID
    6490, 95972
    UniProt
    P40967
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