POLR2A/RPB1 Antikörper (pSer5)

Produktnummer ABIN6938207

Dieses Maus Monoklonal-Antikörper erkennt spezifisch POLR2A/RPB1 in WB, IF, FACS und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für POLR2A/RPB1 Antikörper (pSer5) (ABIN6938207)

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POLR2A/RPB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CTD4H8

Produktdetails anti-POLR2A/RPB1 Antikörper

Bindungsspezifität: pSer5

Spezifität: RNA polymerase II (Pol II) is an enzyme that is composed of 12 subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Ten repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HAP1, K562, PC3, HePG2, NIH3T3 cells. Human testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu POLR2A/RPB1

Target: POLR2A/RPB1 (POLR2A) (RNA Polymerase II Subunit RPB1 (POLR2A))

Andere Bezeichnung: POLR2A

Hintergrund: DNA-directed RNA polymerase II largest subunit RNA polymerase II 220kd subunit, DNA-directed RNA polymerase II subunit RPB1, hRPB220, hsRPB1, POLR2, Polr2a, POLRA, Polymerase (RNA) II (DNA directed) polypeptide A 220 kDa, RNA polymerase II subunit B1, RPBh1, RpIILS, RPO2, RPOL2,RNA Polymerase II
Cellular localisation: Nuclear

Molekulargewicht: 192-253kDa

Gen-ID: 5430, 270017

UniProt: P24928

Pathways: Regulatorische RNA Pathways