Rekombinanter ADRP Antikörper (AA 249-376)

Produktnummer ABIN6938196

Der Kaninchen Monoklonal Anti-ADRP-Antikörper wurde für WB, ELISA, Coat und IHC (f) validiert. Er ist geeignet, ADRP in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter ADRP Antikörper (AA 249-376) (ABIN6938196)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ADRP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: ADFP-2755R

Produktdetails anti-ADRP Antikörper

Bindungsspezifität: AA 249-376

Spezifität: Recognizes a protein of 48 kDa, which is identified as Adipophilin. It belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order Ab without BSA),Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Andere Bezeichnung: PLIN2

Hintergrund: Adipophilin, ADFP, Adipose differentiation-related protein (ADRP), Perilipin-2 (PLIN2),Adipophilin / Perilipin-2 (Marker of Lipid Accumulation)
Cellular localisation: Cytoplasmic

Molekulargewicht: 48kDa

Gen-ID: 123, 3416

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism