Phosphotyrosine Antikörper

Produktnummer ABIN6938185

Der Maus Monoklonal anti-Phosphotyrosine Antikörper (Klon PY265) (ABIN6938185) detektiert spezifisch Phosphotyrosine in WB, IF und FACS.

Kurzübersicht für Phosphotyrosine Antikörper (ABIN6938185)

Target: Phosphotyrosine

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: PY265

Produktdetails anti-Phosphotyrosine Antikörper

Aufreinigung: Purified by Protein A/G

Immunogen: Phosphotyrosine conjugated to BSA

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: MCF-7, MDA-231, T47-D cells. Breast carcinoma.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Andere Bezeichnung: Phosphotyrosine (P-Tyr)

Substanzklasse: Amino Acid

Hintergrund: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.