Prohibitin Antikörper (AA 167-261)

Produktnummer ABIN6938171

Dieser Maus Monoklonal Antikörper detektiert spezifisch Prohibitin in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Prohibitin Antikörper (AA 167-261) (ABIN6938171)

Target: Prohibitin (PHB)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prohibitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: PHB-3231

Produktdetails anti-Prohibitin Antikörper

Bindungsspezifität: AA 167-261

Verwendungszweck: Prohibitin (Mitochondrial Marker) Antibody

Spezifität: Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human PHB protein fragment (aa167-261) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunofluorescence (1-2 μg), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Ramos, Jurkat, HEK293, K562, HepG2, A431 or MCF-7 cells (IF). Liver, Kidney, Heart or Endometrium (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Prohibitin

Target: Prohibitin (PHB)

Andere Bezeichnung: PHB

Hintergrund:

Epididymis luminal protein 215, Epididymis secretory sperm binding protein Li 54e, HEL 215, HEL S 54e, PHB, PHB1, Prohibitin

A431 or MCF-7 cells (IF). Liver, Heart or Endometrium (IHC)., HEK293, HePG2, Jurkat, K562, Kidney, Ramos

Molekulargewicht: 30kDa

Gen-ID: 5245, 514303

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling