CD31 Antikörper
Kurzübersicht für CD31 Antikörper (ABIN6938149)
Target
Alle CD31 (PECAM1) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
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Keine Kreuzreaktivität
- Schwein, Ratte (Rattus)
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Kreuzreaktivität (Details)
- Human. Cynomolgus Monkey. Rabbit. Does not react with Rat or Pig.
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Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Membrane preparation of a spleen from a patient with hairy cell leukemia
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Isotyp
- IgG1 kappa
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Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Tonsil, Angiosarcoma.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Konservierungsmittel
- Azide free
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Haltbarkeit
- 24 months
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- CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))
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Andere Bezeichnung
- PECAM1
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Hintergrund
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EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell surface and cytoplasm of endothelial cells -
Molekulargewicht
- ~100kDa (endothelium) and ~130kDa (platelets)
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Gen-ID
- 5175, 376675, 514412
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UniProt
- P16284
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Pathways
- Regulation of Actin Filament Polymerization
Target
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