Rekombinanter Nuclear Antigen (Pan-Nuclear Marker) Antikörper

Produktnummer ABIN6938095

Produktdetails

Target: Nuclear Antigen (Pan-Nuclear Marker)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)

Spezifität: This MAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Keine Kreuzreaktivität: Schwein

Aufreinigung: Purified by Protein A/G

Immunogen: Nuclei of HL60 cells

Klon: NM2984R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: All human cells. Tonsil.

Known Application: Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 10 mM PBS without BSA and without Azide.

Konservierungsmittel: Azide free

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months.

Haltbarkeit: 24 months

Antigendetails

Target: Nuclear Antigen (Pan-Nuclear Marker)