LIG1 Antikörper

Produktnummer ABIN6937786

Dieses Anti-LIG1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von LIG1 in WB, ELISA und IP. Geeignet für Rind (Kuh).

Kurzübersicht für LIG1 Antikörper (ABIN6937786)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reaktivität: Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIG1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: 1A9

Produktdetails anti-LIG1 Antikörper

Spezifität: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. Furthermore, DNA Ligase I antisense ODNs syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme.

Kreuzreaktivität (Details): Human. Mouse. Rat. Bovine.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Full-length native cow LIG1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), ELISA, Immunoprecipitation,Optimal dilution for a specific application should be determined.

Positive_Control: MOLT-4, PC-3 or Jurkat cell lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Andere Bezeichnung: LIG1

Hintergrund: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1,DNA Ligase 1
Cellular localisation: Nucleus.

Molekulargewicht: 133kDa

Gen-ID: 3978, 1770

UniProt: P18858

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA