KRT8, KRT18 Antikörper

Produktnummer ABIN6937758

Der Maus Monoklonal anti-KRT8, KRT18 Antikörper wird verwendet zum Nachweis von KRT8, KRT18 in Proben von Human, Rind (Kuh), Affe, Schwein und Schaf. Er wurde validiert für IHC, WB, FACS und IF.

Kurzübersicht für KRT8, KRT18 Antikörper (ABIN6937758)

Target: KRT8, KRT18

Reaktivität: Human, Rind (Kuh), Affe, Schwein, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: C-51

Produktdetails

Verwendungszweck: Cytokeratin 8/18 Antibody

Spezifität: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells, there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Keine Kreuzreaktivität: Huhn, Esel, Frettchen, Maus, Kaninchen, Ratte (Rattus), Xenopus laevis

Kreuzreaktivität (Details): Human. Monkey. Cow. Sheep. Pig. Does not react with Mouse, Rat, Ferret, Rabbit, donkey, Chicken or xenopus laevis.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeleton preparation from HeLa cells

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7 or A431 cells. Skin, colon, lung or breast carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: KRT8, KRT18

Andere Bezeichnung: KRT8 & KRT18

Hintergrund:

CARD2, CK8, CYK8, CYKER, Cytokeratin Endo A, DreK8, EndoA, K2C8, K8, Keratin 8, Krt 2.8, KRT8, Type-II Keratin Kb8. Cell Proliferation-inducing Gene 46 Protein, CK18, CYK18Cytokeratin Endo B, K18, Keratin-18, Kerd, KRT18

Colon, lung or breast carcinoma., MCF-7 or A431 cells. Skin

Molekulargewicht: 52.5kDa (CK8), 45kDa (CK18)

Gen-ID: 3856, 3875, 533782

UniProt: P05787, P05783