IGHM Antikörper

Produktnummer ABIN6937512

Der Maus Monoklonal anti-IGHM Antikörper wird verwendet zum Nachweis von IGHM in Proben von Human. Er wurde validiert für IHC, FACS und IF.

Kurzübersicht für IGHM Antikörper (ABIN6937512)

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IGHM Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: ICO-30

Produktdetails anti-IGHM Antikörper

Verwendungszweck: IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody

Spezifität: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Heavy chain of human IgM

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Raji or hPBL cells. Tonsil or Spleen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Andere Bezeichnung: IGHM

Hintergrund:

AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

293T, Raji or hPBL cells. Tonsil or Spleen.

Molekulargewicht: 50-75kDa

Gen-ID: 3507, 510635

UniProt: P01871