Rekombinanter IGHM Antikörper

Produktnummer ABIN6939724

Dieser Maus Monoklonal Antikörper detektiert spezifisch IGHM in IHC, FACS, IF und ELISA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter IGHM Antikörper (ABIN6939724)

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IGHM Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA

Klon: RIGHM-1623

Produktdetails anti-IGHM Antikörper

Verwendungszweck: Recombinant IgM (Immunoglobulin Mu Heavy Chain) (B-Cell Marker) Antibody

Spezifität: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. Monomeric IgM is expressed as a membrane bound antibody on the surface of B cells and as a pentamer when secreted by plasma cells. IgM antibody is prominent in early immune responses to most antigens. Aberrant levels are associated with immune deficiency states, hereditary deficiencies, myeloma, Waldenstrom's macroglobulinemia, chronic infection and hepatocellular disease. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length IGHM protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: 293T, Raji or hPBL cells. Human tonsil or spleen (IHC).

Known Application: ELISA (For coating, order antibody without BSA), ,Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu IGHM

Target: IGHM (Immunoglobulin Heavy Constant mu (IGHM))

Andere Bezeichnung: IGHM

Hintergrund:

AGM1, IGHM, Constant Region of Heavy Chain of IgM, Ig Mu Chain C Region

293T, Raji or hPBL cells. Human tonsil or spleen (IHC).

Molekulargewicht: 50-75kDa

Gen-ID: 3507, 510635

UniProt: P01871