IDH1 Antikörper (AA 281-414)
-
- Target Alle IDH1 Antikörper anzeigen
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
-
Bindungsspezifität
- AA 281-414
-
Reaktivität
- Human
-
Wirt
- Maus
-
Klonalität
- Monoklonal
-
Konjugat
- Dieser IDH1 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- It recognizes a 45 kDa protein, which is identified as isocitrate dehydrogenase (IDH1). It belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 catalyzes the third step of the citric acid cycle, which involves the oxidative decarboxylation of isocitrate, forming i±-ketoglutarate and CO2 in a two-step reaction. The first step involves the oxidation of isocitrate to the intermediate oxalosuccinate, while the second step involves the production of i±-ketoglutarate. During this process, either NADH or NADPH is produced along with CO2. Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma. IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment of human IDH1 protein (around aa 281-414) (exact sequence is proprietary)
- Klon
- IDH1-1152
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product IDH1 Primärantikörper
-
-
- Applikationshinweise
-
Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: HepG2, HeLa, HT29 or MCF7 cells. Human breast, colon or prostate carcinoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
-
- Target
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- Andere Bezeichnung
- IDH1 (IDH1 Produkte)
- Synonyme
- IDCD antikoerper, IDH antikoerper, IDP antikoerper, IDPC antikoerper, PICD antikoerper, NADP-CICDH antikoerper, AI314845 antikoerper, AI788952 antikoerper, E030024J03Rik antikoerper, Id-1 antikoerper, Idh-1 antikoerper, Idpc antikoerper, cb876 antikoerper, fm90e09 antikoerper, im:7143416 antikoerper, wu:fm90e09 antikoerper, F23E12.180 antikoerper, F23E12_180 antikoerper, IDH-I antikoerper, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 antikoerper, isocitrate dehydrogenase 1 antikoerper, isocitrate dehydrogenase I antikoerper, isocitrate dehydrogenase (NADP(+)) 1, cytosolic antikoerper, isocitrate dehydrogenase 1 (NADP+), soluble antikoerper, isocitrate dehydrogenase 1 (NADP+) L homeolog antikoerper, isocitrate dehydrogenase 1 antikoerper, IDH1 antikoerper, Idh1 antikoerper, idh1.L antikoerper, idh1 antikoerper
- Hintergrund
-
Cytosolic NADP-isocitrate dehydrogenase, Epididymis luminal protein 216, Epididymis secretory protein Li 26, HEL-216, HEL-S-26, ICDH, IDCD, IDH1, IDP, IDPC, Isocitrate dehydrogenase 1 (NADP+) soluble, NADP dependent isocitrate dehydrogenase peroxisomal, NADP(+)-specific ICDH, Oxalosuccinate decarboxylase, PICD,IDH1 (Isocitrate Dehydrogenase)
Cellular localisation: Cytoplasm. Nucleus. - Molekulargewicht
- 45-47kDa
- Gen-ID
- 3417, 593422
- UniProt
- O75874
- Pathways
- Warburg Effekt
-