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Rekombinanter ICAM-3/CD50 Antikörper

ICAM3 Reaktivität: Human IHC (f), Bind Wirt: Kaninchen Monoclonal ICAM3-2873R unconjugated Recombinant Antibody
Produktnummer ABIN6937471
  • Target Alle ICAM-3/CD50 (ICAM3) Antikörper anzeigen
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 110
    • 3
    • 2
    • 2
    Human
    Wirt
    • 68
    • 41
    • 1
    Kaninchen
    Klonalität
    • 70
    • 40
    Monoklonal
    Konjugat
    • 58
    • 10
    • 6
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ICAM-3/CD50 Antikörper ist unkonjugiert
    Applikation
    • 61
    • 49
    • 45
    • 34
    • 25
    • 23
    • 22
    • 13
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Binding Studies (Bind)
    Spezifität
    Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.
    Kreuzreaktivität (Details)
    Human.
    Aufreinigung
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Recombinant full-length human ICAM3 protein
    Klon
    ICAM3-2873R
    Isotyp
    IgG
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  • Applikationshinweise

    Known_Application: Blocks binding of CD11a (LFA-1) to CD50 (ICAM-3) (Order Ab without Azide), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: HL-60 or THP-1 cells. Lymph node and tonsil.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Haltbarkeit
    24 months
  • Target
    ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))
    Andere Bezeichnung
    ICAM3 (ICAM3 Produkte)
    Synonyme
    CD50 antikoerper, CDW50 antikoerper, ICAM-R antikoerper, ICAM-3 antikoerper, ICAM3 antikoerper, intercellular adhesion molecule 3 antikoerper, ICAM3 antikoerper, Icam3 antikoerper
    Hintergrund
    CD50, CDW 50, CDw50, ICAM 3, ICAM-3, ICAM-R, ICAM3, ICAM3_HUMAN, ICAMR, Intercellular adhesion molecule 3,CD50 / ICAM3
    Cellular localisation: Membrane.
    Molekulargewicht
    110-160kDa
    Gen-ID
    3385, 654563
    UniProt
    P32942
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