HSPG2 Antikörper

Produktnummer ABIN6937452

Der Ratte Monoklonal Anti-HSPG2-Antikörper wurde für IF, FACS und IHC (f) validiert. Er ist geeignet, HSPG2 in Proben von Maus zu detektieren.

Kurzübersicht für HSPG2 Antikörper (ABIN6937452)

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser HSPG2 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: A7L6

Produktdetails anti-HSPG2 Antikörper

Spezifität: This MAb specifically precipitates heterogeneous material of high MW, identified as perlecan, a major heparan-sulfate proteoglycan (HSPG) within all basement membranes and cell surfaces. It does not cross-react with laminin, fibronectin, or dermatran sulfate proteoglycan. Because of perlecan's strategic location and ability to store and protect growth factors, it has been strongly implicated in the control of tumor cell growth and metastatic behavior. Perlecan possesses angiogenic and growth-promoting attributes primarily by acting as a co-receptor for basic fibroblast growth factor (FGF-2). Suppression of perlecan causes substantial inhibition of neoplastic growth and neovascularization. Thus, perlecan is a potent inducer of neoplasm growth and angiogenesis in vivo and therapeutic interventions targeting this key modulator of tumor progression may improve neoplastic treatment.

Kreuzreaktivität (Details): Human. Monkey. Cow. Pig. Mouse. Fish.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Murine EHS laminin preparation

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Breast carcinomas, squamous cell carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu HSPG2

Target: HSPG2 (Heparan Sulfate Proteoglycan 2 (HSPG2))

Andere Bezeichnung: HSPG2

Hintergrund: Heparan sulfate proteoglycan of basement membrane, HSPG, Hspg2, LG3 peptide, Perlecan, PLC, SJS1,Heparan Sulfate Proteoglycan (Large) / Perlecan
Cellular localisation: Basement membrane

Molekulargewicht: >400kDa

Gen-ID: 3339, 562227

UniProt: P98160

Pathways: Glycosaminoglycan Metabolic Process, Lipid Metabolism