FTH1 Antikörper (AA 58-180)

Produktnummer ABIN6937271

Produktdetails anti-FTH1 Antikörper

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Bindungsspezifität: AA 58-180

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FTH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Mammalian ferritins consist of 24 subunits made up of 2 types of poly-peptide chains, ferritin heavy chain and ferritin light chain, which each have unique functions. Ferritin heavy chains catalyze the first step in iron storage, the oxidationof FeI(II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe(III). The most prominent role of mammalian ferritins is to provide iron-buffering capacity to cells. In addition to iron buffering, heavy chain ferritin is also involved in the regulation of thymidine biosynthesis via increased expression of cytoplasmic serine hydroxymethyltransferase, which is a limiting factor in thymidylate synthesis in MCF-7 cells. Light chain ferritin is involved in cataracts by at least two mechanisms: hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of aging-related cataracts.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human FTH1 protein fragment (around aa 58-180) (exact sequence is proprietary)

Klon: FTH-2081

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.1-0.2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HepG2, HeLa, HL-60 or 293T cells. Pancreas, Liver, Cerebellum or Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu FTH1

Target: FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))

Andere Bezeichnung: FTH1 (FTH1 Produkte)

Hintergrund: Apoferritin, Cell proliferation-inducing gene 15 protein, Ferritin H subunit, Ferritin heavy chain, FHC, FRIH, FTH1, FTHL6, Iron overload autosomal dominant, PIG15, Placenta immunoregulatory factor, Proliferation inducing gene 15 protein (PLIF),Ferritin, Heavy Chain (FTH) (Microglia Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 21kDa

Gen-ID: 2495, 524910, 645560

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis