ERBB4 Antikörper (AA 1116-1269)

Produktnummer ABIN6937181

Dieses Maus Monoklonal-Antikörper erkennt spezifisch ERBB4 in IF und FACS. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ERBB4 Antikörper (AA 1116-1269) (ABIN6937181)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERBB4 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: HFR-1

Produktdetails anti-ERBB4 Antikörper

Bindungsspezifität: AA 1116-1269

Spezifität: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis.

Kreuzreaktivität (Details): Human. Mouse. Predicted to work with Rat, Chicken.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Breast, brain, heart or kidney. MCF-7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ERBB4

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Andere Bezeichnung: ERBB4

Hintergrund: Avian erythroblastic leukemia viral oncogene homolog 4, ERBB4, HER4, human epidermal growth factor receptor 4, p180erbB4, Proto-oncogene-like protein c-ErbB-4,HER-4 / ERBB4
Cellular localisation: Cytoplasmic, Cell Surface and Nuclear

Molekulargewicht: 180kDa (precursor), 80/120kDa (cleaved)

Gen-ID: 2066, 390729

UniProt: Q15303

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung