Chromogranin A Antikörper

Produktnummer ABIN6936985

Dieses Anti-Chromogranin A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Chromogranin A in WB und IHC (f). Geeignet für Human.

Kurzübersicht für Chromogranin A Antikörper (ABIN6936985)

Target: Chromogranin A (CHGA)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Chromogranin A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CHGA-777

Produktdetails anti-Chromogranin A Antikörper

Spezifität: Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Keine Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CHGA protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Adrenal gland, bowel, thyroid, pancreas or pheochromocytoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Chromogranin A

Target: Chromogranin A (CHGA)

Andere Bezeichnung: CHGA

Hintergrund: Beta-Granin, CGA, CHGA, Chromogranin A Parathyroid Secretory Protein 1, ER-37, Pancreastatin, Parastatin, Pituitary Secretory Protein I, SP-I, Vasostatin I or II,Chromogranin A / CHGA (Neuroendocrine Marker)
Cellular localisation: Finely granular cytoplasmic

Molekulargewicht: 68-75kDa

Gen-ID: 1113, 150793

UniProt: P10645

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling