Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

CEACAM1/5 Antikörper

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CEACAM1/5 in FACS, IHC (f) und IF. Er zeigt eine Reaktivität gegenüber Human.
Produktnummer ABIN6936929

Kurzübersicht für CEACAM1/5 Antikörper (ABIN6936929)

Target

CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))

Reaktivität

Human

Wirt

  • 7
  • 5
Maus

Klonalität

  • 10
  • 2
Monoklonal

Konjugat

  • 11
  • 1
Dieser CEACAM1/5 Antikörper ist unkonjugiert

Applikation

  • 6
  • 6
  • 6
  • 4
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunofluorescence (IF)

Klon

C66-195
  • Spezifität

    This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb reacts with nonspecific cross-reacting antigen (NCA) and shows a cross-reaction with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

    Kreuzreaktivität (Details)

    Human and Monkey.

    Aufreinigung

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogen

    Purified human CEA protein

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Positive_Control: MCF7 or 293T cells. Human colon carcinoma

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Konservierungsmittel

    Azide free

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Haltbarkeit

    24 months
  • Target

    CEACAM1/5 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1/5 (CEACAM1/5))

    Andere Bezeichnung

    CEACAM1 & CEACAM5

    Hintergrund

    Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1),Carcinoembryonic Antigen (CEA) / CD66
    Cellular localisation: Cytoplasmic and luminal surface

    Molekulargewicht

    80-200kDa

    Gen-ID

    1048, 709196

    UniProt

    P06731
Sie sind hier:
Chat with us!