CD59 Antikörper

Produktnummer ABIN6936764

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CD59 in FACS, IF und Func. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD59 Antikörper (ABIN6936764)

Target: CD59

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD59 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Klon: 193-27

Produktdetails anti-CD59 Antikörper

Spezifität: Reacts with human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein (Workshop VI, Code N-L036). CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb recognizes CD59 transfected cells. It is useful for study on GPI-anchored proteins, PNH and CD59 functions.

Keine Kreuzreaktivität: Pavian, Pferd

Kreuzreaktivität (Details): Human. Does not react with Baboon or Horse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or Raji cells. Human lymphocytes. Human lymph node and tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD59

Target: CD59

Andere Bezeichnung: CD59

Hintergrund: 20 kDa homologous restriction factor (HRF20), Complement regulatory protein, Human leukocyte antigen MIC11, MAC-inhibitory protein (MACIP), Membrane attack complex inhibition factor (MACIF), Membrane inhibitor of reactive lysis, MIRL, MSK21, Protectin, T cell activating protein,CD59 / Complement Regulatory Protein / Protectin
Cellular localisation: Cell Surface

Molekulargewicht: 20kDa

Gen-ID: 966, 278573, 709466

UniProt: P13987

Pathways: Komplementsystem