CD53 Antikörper

Produktnummer ABIN6936756

Der Maus Monoklonal Anti-CD53-Antikörper wurde für FACS, IF, Func und IM validiert. Er ist geeignet, CD53 in Proben von Human zu detektieren.

Kurzübersicht für CD53 Antikörper (ABIN6936756)

Target: CD53

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD53 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func), Immunomicroscopy (IM)

Klon: 161-2

Produktdetails anti-CD53 Antikörper

Spezifität: Recognizes a protein of 33-55 kDa, identified as CD53 (Workshop VI, Code N-L033). It is expressed on monocytes and macrophages, dendritic cells, osteoblasts and osteoclasts, and on T and B cells from every stage of differentiation but is absent from platelets, red blood cells. CD53 appears to be the marker with widest reactivity as well as the marker with the strictest specificity to hematopoietic cells. CD53 is a type III membrane with both termini in the cytoplasm and two loops in the extracellular environment. This molecule, in common with other members of tetraspan family, is involved in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. CD53 crosslinking induces calcium flux on human monocyte and B cells. Cross-linking of CD53 promotes activation of resting human B-lymphocytes. This MAb recognizes CD53 transfected cells and partially inhibits T-cell proliferation induced by CD3 antibody (clone: UCHT1).

Keine Kreuzreaktivität: Pavian, Pferd

Kreuzreaktivität (Details): Human. Does not react with Baboon or Horse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Stimulated human leukocytes

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Functional Studies (Order Ab without Azide),Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD53

Target: CD53

Andere Bezeichnung: CD53

Hintergrund: CD53 glycoprotein, CD53 tetraspan antigen, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, Tetraspanin-25 (Tspan-25), TSPAN25,CD53 (TSPAN25)
Cellular localisation: Cell Surface

Molekulargewicht: 33-55kDa

Gen-ID: 963, 443057

UniProt: P19397