CD5 Antikörper

Produktnummer ABIN6936748

Dieses Anti-CD5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD5 in ELISA, IHC (f) und Coat. Geeignet für Human.

Kurzübersicht für CD5 Antikörper (ABIN6936748)

Target: CD5

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD5 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Coating (Coat)

Klon: C5-473-CD5-54-F6

Produktdetails anti-CD5 Antikörper

Spezifität: Recognizes a 67 kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95 % of thymocytes and 72 % of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10 %) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.

Kreuzreaktivität (Details): Human. Rabbit. Cow. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human CD5 recombinant protein (C5/473), A synthetic peptide from the intracellular region of human CD5 (CD5/54/F6)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: 293T, Ramos or MOLT-4 cells. Tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD5

Target: CD5

Andere Bezeichnung: CD5

Hintergrund: CD5 antigen (p56 62), LEU1, Ly12, LyA, Lymphocyte antigen T1/Leu-1, Lymphocyte glycoprotein T1/Leu1, T-cell surface glycoprotein CD5,CD5 (Mantle Cell Lymphoma Marker)
Cellular localisation: Cell Surface

Molekulargewicht: 67kDa

Gen-ID: 921, 58685

UniProt: P06127