CD3 epsilon Antikörper (Cytoplasmic Domain)

Produktnummer ABIN6936685

Produktdetails anti-CD3 epsilon Antikörper

Target: CD3 epsilon (CD3E)

Bindungsspezifität: AA 156-168, Cytoplasmic Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Kreuzreaktivität (Details): Human, Rat, Guinea pig, Rabbit, Pig, Horse, Cow and Chicken. Others not known.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: A synthetic peptide corresponding to amino acids 156-168 of the cytoplasmic domain of human CD3- chain

Klon: PC3-188A

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), ,Immunofluorescence (1-2 μg/mL), ,Western Blotting (1-2 μg/mL for 60 minutes at RT),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Tonsil and lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3E (CD3E Produkte)

Hintergrund: CD 3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex,CD3e (T-Cell Marker)
Cellular localisation: Cell Surface and Cytoplasmic

Molekulargewicht: 20kDa

Gen-ID: 916, 3003

UniProt: P07766

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway