C4A Antikörper

Produktnummer ABIN6936544

Der Maus Monoklonal anti-C4A Antikörper (Klon C4D204) (ABIN6936544) detektiert spezifisch C4A in IHC, ELISA und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für C4A Antikörper (ABIN6936544)

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C4A Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Klon: C4D204

Produktdetails anti-C4A Antikörper

Verwendungszweck: Complement 4d (C4d) (Acute Humoral Rejection Marker) Antibody

Spezifität: This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human Complement 4d protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Rejected Renal Transplant Tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Andere Bezeichnung: C4A

Hintergrund:

Acidic Complement C4, Basic C4, C4-1, C4a Anaphylatoxin, C4A, C4A2, C4A3, C4A4, C4A6, C4b, C4B1, C4B12, C4d fragment, C4F, C4S, Complement C4 Gamma Chain, Complement Component 4A, Complement Component 4B

Rejected Renal Transplant Tissue.

Molekulargewicht: 192kDa (predicted)

Gen-ID: 720, 721, 534847

Pathways: Komplementsystem