C4A Antikörper

Produktnummer ABIN6936544

Dieses Anti-C4A-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von C4A in ELISA, Coat und IHC (f). Geeignet für Human.

Kurzübersicht für C4A Antikörper (ABIN6936544)

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser C4A Antikörper ist unkonjugiert

Applikation: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: C4D204

Produktdetails anti-C4A Antikörper

Spezifität: This MAb is specific to Complement 4d (C4d) and reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 Molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human Complement 4d protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating use Ab at 1-2 μg/mL order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1 mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: Rejected Renal Transplant Tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu C4A

Target: C4A (C4a) (Complement Fragment C4a (C4a))

Andere Bezeichnung: C4A

Hintergrund: Acidic Complement C4, Basic C4, C4-1, C4a Anaphylatoxin, C4A, C4A2, C4A3, C4A4, C4A6, C4b, C4B1, C4B12, C4d fragment, C4F, C4S, Complement C4 Gamma Chain, Complement Component 4A, Complement Component 4B,Complement 4d (C4d) (Acute Humoral Rejection Marker)
Cellular localisation: Intracytoplasmic vacuoles of endothelial cells and Secreted

Molekulargewicht: 192kDa (predicted)

Gen-ID: 720, 721, 534847

Pathways: Komplementsystem