Rekombinanter BrdU Antikörper

Produktnummer ABIN6936519

Der Kaninchen Monoklonal anti-BrdU Antikörper wird verwendet zum Nachweis von BrdU in Proben von Diverse Spezies. Er wurde validiert für IHC.

Kurzübersicht für Rekombinanter BrdU Antikörper (ABIN6936519)

Target: BrdU (Bromodeoxyuridine (BrdU))

Antikörpertyp: Recombinant Antibody

Reaktivität: Diverse Spezies

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: BRD2888R

Produktdetails anti-BrdU Antikörper

Verwendungszweck: Recombinant Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody

Spezifität: It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Bromodeoxyuridine (BrdU) conjugated to KLH

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Known Application: Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1 mg/mL PBS, 10 min at 37oC (Cat. #AP-9008)]Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: Bromodeoxyuridine (BrdU) (Proliferation Marker)

Substanzklasse: Chemical

Hintergrund:

Bromodeoxyuridine, BUdr

Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU.

Molekulargewicht: 307Da