Iba1 Antikörper (AA 1-146)

Produktnummer ABIN6936372

Der Maus Monoklonal anti-Iba1 Antikörper wird verwendet zum Nachweis von Iba1 in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für Iba1 Antikörper (AA 1-146) (ABIN6936372)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Iba1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: AIF1-2493

Produktdetails anti-Iba1 Antikörper

Bindungsspezifität: AA 1-146

Verwendungszweck: AIF1 / Iba1 (Microglia Marker) Antibody

Spezifität: AIF1 is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC).

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)

Isotyp: IgG2c, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Lymph Node, Tonsil or Kidney.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: AIF1

Hintergrund:

Allograft inflammatory factor 1 (AIF1), balloon angioplasty responsive transcription (BART1), IBA1, Interferon responsive transcript 1, Ionized calcium-binding adapter molecule 1, IRT1, Microglia response factor (MRF1)

lymph node, Tonsil or Kidney.

Molekulargewicht: 17kDa

Gen-ID: 199, 76364

UniProt: P55008

Pathways: Smooth Muscle Cell Migration