CD31 Antikörper (Extracellular Domain)

Produktnummer ABIN6934749

Der Maus Monoklonal Anti-CD31 Antikörper wurde für WB und IHC (f) validiert. Er ist geeignet, CD31 in Proben von Human zu detektieren.

Kurzübersicht für CD31 Antikörper (Extracellular Domain) (ABIN6934749)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD31 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 1A10

Produktdetails anti-CD31 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Kreuzreaktivität (Details): Human.

Immunogen: Prokaryotic recombinant fusion protein corresponding to a portion of the extracellular domain downstream of the signal sequence of the CD31 (PECAM-1)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1:50-1:100 for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Positive_Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Tissue culture supernatant with 0.05% Azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Andere Bezeichnung: PECAM1

Hintergrund: EndoCAM, PECA1, Platelet Endothelial Cell Adhesion Molecule 1, GPIIA',CD31 / PECAM-1 (Endothelial Cell Marker)
Cellular localisation: Cell surface and cytoplasm of endothelial cells

Molekulargewicht: ~100kDa (endothelium) and ~130kDa (platelets)'

Gen-ID: 5175, 376675, 514412

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization