Nucleolin Antikörper (CF®488A)

Produktnummer ABIN6934519

Dieses Anti-Nucleolin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Nucleolin in IF, FACS, ISt, StM und T. Geeignet für Human.

Kurzübersicht für Nucleolin Antikörper (CF®488A) (ABIN6934519)

Target: Nucleolin (NCL)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Nucleolin Antikörper ist konjugiert mit CF®488A

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Staining Methods (StM), Test (T)

Klon: NCL-902

Produktdetails anti-Nucleolin Antikörper (CF®488A)

Spezifität: Recognizes a protein of ~76 kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.This MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Keine Kreuzreaktivität: Rind (Kuh), Maus, Ratte (Rattus)

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant human NCL protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HeLa cells. Breast Cancer.

Known Application: Flow Cytometry (5 µL per test per one million cells in 0.1 mL or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Nucleolin

Target: Nucleolin (NCL)

Andere Bezeichnung: NCL

Molekulargewicht: 76kDa

Gen-ID: 4691

UniProt: P19338

Pathways: Ribonucleoprotein Complex Subunit Organization