IGHA1 & IGHA2 Antikörper (CF®488A)

Produktnummer ABIN6933799

Produktdetails

Target: IGHA1 & IGHA2

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®488A

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Staining Methods (StM)

Spezifität: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Alpha heavy chain. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Aufreinigung: Purified by Protein A/G

Immunogen: Purified human alpha heavy chain

Klon: IA761

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (1-2 µL/million cells), Immunofluorescence (0.5-1.0 μg/mL 30 min RT),Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: IGHA1 & IGHA2

Molekulargewicht: 50-75kDa

Gen-ID: 3493, 3494

UniProt: P01876, P01877