IGHA1 & IGHA2 Antikörper

Produktnummer ABIN6939703

Dieser Maus Monoklonal Antikörper detektiert spezifisch IGHA1 & IGHA2 in IHC, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für IGHA1 & IGHA2 Antikörper (ABIN6939703)

Target: IGHA1 & IGHA2

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: HISA43

Produktdetails

Verwendungszweck: IgA (Immunoglobulin Alpha Heavy Chain) (B-Cell Marker) Antibody

Spezifität: This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with both IgA1 and IgA2 subclasses of Alpha heavy chain. It reacts with the third constant domain (CH3) of the alpha chain of IgA molecules. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Purified human IgA

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (0.5-1.0 μg/mL 30 min RT), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL 30 min RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: IGHA1 & IGHA2

Hintergrund:

A2m Marker, Ig alpha 1 Chain C Region, Ig alpha 2 Chain C Region, IGHA1, IGHA2, Immunoglobulin Am1, Immunoglobulin Am2, Immunoglobulin Heavy Constant Alpha 1, Immunoglobulin Heavy Constant Alpha 2

293T, Daudi, Raji or hPBL cells. Tonsil or Spleen.

Molekulargewicht: 50-75kDa

Gen-ID: 3493, 3494, 699841

UniProt: P01876