GRB2 Antikörper (Cy5)

Produktnummer ABIN686936

Der Kaninchen Polyklonal Anti-GRB2-Antikörper wurde für IF (p) validiert. Er ist geeignet, GRB2 in Proben von Human, Maus, Ratte, Hund und Huhn zu detektieren.

Kurzübersicht für GRB2 Antikörper (Cy5) (ABIN686936)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reaktivität: Human, Maus, Ratte, Hund, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GRB2 Antikörper ist konjugiert mit Cy5

Applikation: Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Produktdetails anti-GRB2 Antikörper (Cy5)

Spezifität: Excitation / Emission Wavelengths: 650nm/670nm

Kreuzreaktivität: Ratte (Rattus), Huhn, Hund

Aufreinigung: purified by Protein A

Immunogen: KLH conjugated synthetic peptide derived from human GRB2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P)(1:100-500)
Optimal working dilution should be determined by the investigator.

Kommentare:

Excitation / Emission wavelength: 652nm / 670nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 µg/µL

Buffer: Aqueous buffered solution containing 100 µg/mL BSA, 50 % glycerol and 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4 °C for 12 months

Haltbarkeit: 12 months

Details zu GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Andere Bezeichnung: GRB2/ASH

Hintergrund: Growth factor receptor bound protein 2 (GRB2), also known as Ash protein, is a 24 kDa protein that contains a central Src homology (SH2) domain flanked by two SH3 domains. GRB2 is believed to be a regulatory subunit of signaling molecules whose activity is modulated by receptor binding. GRB2 associates with activated (tyrosine phosphorylated) EGFR and PDGFR via its SH2 domain as well as IRS1, SHC, and LNK through SH2 and SH3 domains. The SH3 domain binds to SOS, a guanine nucleotide exchange factor for Ras proteins. Endocytosis of activated EGFR requires the interaction of GRB2 with the GTP binding protein dynamin, a factor essential to the formation of endocytotic vesicle. It is ubiquitously expressed and several isoforms are produced by alternative splicing. On SDS PAGE, GRB2 has an apparent molecular weight of 28 kDa.

Molekulargewicht: 25kDa

Gen-ID: 2885

Pathways: RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation