AIF1 Antikörper (Allograft Inflammatory Factor 1) (AA 116-147)

Details for Product anti-AIF1 Antibody No. ABIN685477, Anbieter: Anmelden zum Anzeigen
Antigen
  • fa04b11
  • wu:fa04b11
  • AIF1
  • aif1
  • AIF-1
  • IBA1
  • IRT-1
  • IRT1
  • Bart1
  • iba1
  • mrf-1
  • AI607846
  • D17H6S50E
  • G1
  • Iba1
  • AIF
  • AIF1L
  • allograft inflammatory factor 1
  • allograft inflammatory factor 1-like
  • aif1
  • AIF1
  • Aif1
  • C9ORF58
Epitop
AA 116-147
38
24
19
16
15
14
9
8
8
8
7
6
4
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
224
108
105
9
7
2
2
1
1
Wirt
Kaninchen
153
59
43
2
1
Klonalität
Polyklonal
Konjugat
Dieser AIF1 Antikörper ist unkonjugiert
29
25
12
5
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
201
130
126
74
29
26
19
18
16
12
10
4
2
2
2
2
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Chargennummer 980993W
Validierte Anwendung Immunofluorescence
Positivkontrolle Mouse spleen
Negativkontrolle Mouse duodenum
Bewertung Strong signal was observed in the positive control tissue, while no signal was observed in the negative control tissue.
Primärantikörper
Isotypkontrolle
Sekundärantikörper
Kontrollen
  • Positive control: Wild type mouse spleen (specimen known to contain the target protein) from Explora BioLabs.
  • Negative Control: Wild type mouse duodenum (specimen known to not contain the target protein) from Explora BioLabs.
  • Primary antibody isotype control: Wild type mouse spleen treated with primary antibody isotype control instead of the primary antibody.
  • Secondary antibody only control: Wild type mouse spleen treated with secondary antibody only (no primary antibody).
Protokoll
  • Frozen, OCT embedded tissues were cut 5 µm and mounted on positive charged slides.
  • Sections were air dried for 30 min prior to fix in 10% neutral buffered formalin for 10 min.
  • Fixed slides were rinsed three times in PBS for 5 min each at RT.
  • Sections were blocked in 1 X PBS / 3% donkey serum / 0.1% Triton X-100 for 10 min at RT.
  • Sections were incubated with primary antibody diluted 1:100 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) overnight at 4°C.
  • Sections were rinsed three times in PBS for 5 min each at RT.
  • Sections were incubated with secondary antibody diluted 1:200 in Universal Antibody Dilution Buffer (Electron Microscopy Sciences, 25886-05) for 120 min at RT in dark.
  • Sections were mounted on slides with FluoroGel II with DAPI (Electron Microscopy Sciences).
  • Stained sections were imaged with a 3D Histech Pannoramic SCAN 150.
Anmerkungen None
Validierungsbilder
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 1: AIF1 staining on mouse spleen (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 2: AIF1 staining on mouse duodenum (red), counterstained with DAPI (blue).
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 3: Isotype control staining on mouse spleen (red), counterstained with DAPI (b...
Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Figure 4: Secondary only staining on mouse spleen (red), counterstained with DAPI (bl...
Immunogen KLH conjugated synthetic peptide derived from human AIF1
Isotyp IgG
Reinigung Purified by Protein A.
Plasmids, Primers & others Plasmide, Primers & weitere AIF1 products on genomics-online (e.g. as negative or positive controls)
Andere Bezeichnung Aif1 (AIF1 Antibody Abstract)
Hintergrund

Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

Subcellular location: Cytoplasm, Membrane bound

Synonyms: IBA1, IRT1, AIF-1, IRT-1, Allograft inflammatory factor 1, Ionized calcium-binding adapter molecule 1, Protein G1, AIF1, G1

Gen-ID 199
UniProt P55008
Pathways Smooth Muscle Cell Migration
Applikations-hinweise WB 1:100-1000
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store at -20°C
Haltbarkeit 12 months
Bilder des Herstellers
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclo...
Immunohistochemistry (IHC) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclona...
Immunofluorescence (IF) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Independently Validated Antibody, image provided by Science Direct, badge number 0296...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclon...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclon...
 image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) BV-microglia stained with AIF1 Polyclonal Antibody at 1:100 dilution. AIF1 expressio...
 image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 116-147) antibody (ABIN685477) Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal A...
Produkt verwendet in: Ko, Shie, Lin, Chen, Yao, Chen: "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." in: Scientific reports, Vol. 7, Issue 1, pp. 17489, 2018 (PubMed). Von den Autoren verwendete Methode: Immunofluorescence (fixed cells) (IF/ICC) (Probematerial (Species): Rat (Rattus)).

Hsu, Yao, Hsu, Lin, Chen, Chen: "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." in: Scientific reports, Vol. 7, pp. 42280, 2017 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Probematerial (Species): Rat (Rattus)). Weitere Details: Sample: spinal cord

Fu, Xia, Liu, Wang, Wang, Shi, Song, Song, Song et al.: "The acute exposure of tetrachloro-p-benzoquinone (a.k.a. chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin ..." in: Toxicology, Vol. 381, pp. 39-50, 2017 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Mouse (Murine)). Weitere Details: Sample: brain

Hu, Kodithuwakku, Zhou, Li, Han, Fang, Liu, Li: "Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 Pathway." in: Molecules (Basel, Switzerland), Vol. 22, Issue 6, 2017 (PubMed). Von den Autoren verwendete Methode: Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (Probematerial (Species): Mouse (Murine)).

Ogawa, Onozato, Okuhara, Nagasawa, Tamura, Hayashi: "Spontaneous cutaneous soft tissue sarcoma with differentiation into fibroblasts in a Sprague-Dawley rat." in: Journal of toxicologic pathology, Vol. 29, Issue 2, pp. 119-24, 2016 (PubMed). Von den Autoren verwendete Methode: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Probematerial (Species): Rat (Rattus)).

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